Stark, W. M. (2017) Making serine integrases work for us. Current Opinion in Microbiology, 38, pp. 130-136. (doi: 10.1016/j.mib.2017.04.006) (PMID:28599144)
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Abstract
DNA site-specific recombinases are enzymes (often associated with mobile DNA elements) that catalyse breaking and rejoining of DNA strands at specific points, thereby bringing about precise genetic rearrangements. Serine integrases are a group of recombinases derived from bacteriophages. Their unusual properties, including directionality of recombination and simple site requirements, are leading to their development as efficient, versatile tools for applications in experimental biology, biotechnology, synthetic biology and gene therapy. This article summarizes our current knowledge of serine integrase structure and mechanism, then outlines key factors that affect the performance of these phage recombination systems. Recently published studies, that have expanded the repertoire of available systems and reveal system-specific characteristics, will help us to choose the best integrases for envisaged applications.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Stark, Professor Marshall |
| Authors: | Stark, W. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Current Opinion in Microbiology |
| Publisher: | Elsevier |
| ISSN: | 1369-5274 |
| ISSN (Online): | 1879-0364 |
| Published Online: | 06 June 2017 |
| Copyright Holders: | Copyright © 2017 Crown Copyright |
| First Published: | First published in Current Opinion in Microbiology 38: 130-136 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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