Integrated immunoassay using tuneable surface acoustic waves and lensfree detection

Bourquin, Y., Reboud, J. , Wilson, R. , Zhang, Y. and Cooper, J. M. (2011) Integrated immunoassay using tuneable surface acoustic waves and lensfree detection. Lab on a Chip, 11(16), p. 2725. (doi:10.1039/C1LC20320G) (PMID:21725557)

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Abstract

The diagnosis of infectious diseases in the Developing World is technologically challenging requiring complex biological assays with a high analytical performance, at minimal cost. By using an opto-acoustic immunoassay technology, integrating components commonly used in mobile phone technologies, including surface acoustic wave (SAW) transducers to provide pressure driven flow and a CMOS camera to enable lensfree detection technique, we demonstrate the potential to produce such an assay. To achieve this, antibody functionalised microparticles were manipulated on a low-cost disposable cartridge using the surface acoustic waves and were then detected optically. Our results show that the biomarker, interferon-γ, used for the diagnosis of diseases such as latent tuberculosis, can be detected at pM concentrations, within a few minutes (giving high sensitivity at a minimal cost).

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wilson, Dr Robert and Cooper, Professor Jonathan and Reboud, Dr Julien
Authors: Bourquin, Y., Reboud, J., Wilson, R., Zhang, Y., and Cooper, J. M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Lab on a Chip
Publisher:Royal Society of Chemistry
ISSN:1473-0197
ISSN (Online):1473-0189
Published Online:01 July 2011

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
465001Listening to the Microworld.Jonathan CooperEngineering and Physical Sciences Research Council (EPSRC)EP/F040857/1ENG - BIOMEDICAL ENGINEERING