Biomarkers and bacteria around implants and natural teeth in the same individuals

Gürlek, Ö., Gümüş, P., Nile, C. J. , Lappin, D. F. and Buduneli, N. (2017) Biomarkers and bacteria around implants and natural teeth in the same individuals. Journal of Periodontology, 88(8), pp. 752-761. (doi: 10.1902/jop.2017.160751) (PMID:28440740)

Abstract

Aim: This cross-sectional study assessed cytokine levels in peri-implant crevicular fluid (PICF)/ gingival crevicular fluid (GCF) and a selection of subgingival/submucosal plaque bacteria from clinically healthy or diseased sites in the same individuals. Material and Methods: Samples from 97 implants/teeth (58 implants: 19 healthy, 20 mucositis, 19 peri-implantitis; 39 natural teeth: 19 healthy, 12 gingivitis, 8 periodontitis) in 15 systemically healthy patients were investigated by immunoassay, real-time PCR. Samples were obtained first and then probing depth, clinical attachment level, bleeding on probing, plaque index scores, keratinized tissue width were recorded. Data were analyzed by Wilcoxon, Mann-Whitney and permutation tests on dependent, independent, mixed dependent and independent samples and Spearman correlation. Results: Interleukin-1beta levels were significantly higher in PICF samples of healthy implants than in GCF samples of healthy teeth (p=0.003), soluble activator of nuclear factor Kappa-B (sRANKL) concentrations were significantly higher in gingivitis than mucositis group (p=0.004). The biomarker levels were similar in peri-implantitis and periodontitis groups (p>0.05). Actinomyces naeslundi and Streptococcus oralis levels were significantly higher in healthy implant group than healthy teeth (p<0.05). Prevotella intermedia and Treponema denticola levels were lower in mucositis group than in gingivitis group (p<0.05). Prevotella oralis and S. oralis levels were significantly higher in the periodontitis group (p<0.05) and T. denticola levels were significantly higher in the peri-implantitis group (p<0.05). Conclusion: There were many similarities but crucially some differences in biomarker levels (IL-1β and sRANKL) and bacterial species between peri-implant and periodontal sites in the same individuals suggesting similar pathogenic mechanisms.

• Item Type: Articles
• Additional Information: This study was supported by a grant from the Ege University Research Foundation (Project No: 2014 DIS 018).
• Keywords: Biomarkers, cytokines, dental implant, peri-implantitis, periodontitis
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Lappin, Dr David and Nile, Dr Christopher
• Authors: Gürlek, Ö., Gümüş, P., Nile, C. J., Lappin, D. F., and Buduneli, N.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
• Journal Name: Journal of Periodontology
• Publisher: American Academy of Periodontology
• ISSN: 0022-3492
• ISSN (Online): 1943-3670
• Copyright Holders: Copyright © 2017 American Academy of Periodontology
• First Published: First published in Journal of Periodontology 88(8): 752-761
• Publisher Policy: Reproduced in accordance with the publisher copyright policy