RNA interference restricts rift valley fever virus in multiple insect systems

Dietrich, I. et al. (2017) RNA interference restricts rift valley fever virus in multiple insect systems. mSphere, 2(3), 00090-17. (doi:10.1128/mSphere.00090-17) (PMID:28497117) (PMCID:PMC5415632)

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Abstract

The emerging bunyavirus Rift Valley fever virus (RVFV) is transmitted to humans and livestock by a large number of mosquito species. RNA interference (RNAi) has been characterized as an important innate immune defense mechanism used by mosquitoes to limit replication of positive-sense RNA flaviviruses and togaviruses; however, little is known about its role against negative-strand RNA viruses such as RVFV. We show that virus-specific small RNAs are produced in infected mosquito cells, in Drosophila melanogaster cells, and, most importantly, also in RVFV vector mosquitoes. By addressing the production of small RNAs in adult Aedes sp. and Culex quinquefasciatus mosquitoes, we showed the presence of virus-derived Piwi-interacting RNAs (piRNAs) not only in Aedes sp. but also in C. quinquefasciatus mosquitoes, indicating that antiviral RNA interference in C. quinquefasciatus mosquitoes is similar to the described activities of RNAi in Aedes sp. mosquitoes. We also show that these have antiviral activity, since silencing of RNAi pathway effectors enhances viral replication. Moreover, our data suggest that RVFV does not encode a suppressor of RNAi. These findings point toward a significant role of RNAi in the control of RVFV in mosquitoes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Fall, Dr Gamou and Dietrich, Dr Isabelle and Elliott, Professor Richard and Schnettler, Dr Esther and Brennan, Dr Benjamin and Kohl, Professor Alain
Authors: Dietrich, I., Jansen, S., Fall, G., Lorenzen, S., Rudolf, M., Huber, K., Heitmann, A., Schicht, S., Ndiaye, E. H., Watson, M., Castelli, I., Brennan, B., Elliott, R. M., Diallo, M., Sall, A. A., Failloux, A.-B., Schnettler, E., Kohl, A., Becker, S. C., and Duprex, W. P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:mSphere
Publisher:American Society for Microbiology
ISSN:2379-5042
ISSN (Online):2379-5042
Published Online:03 May 2017
Copyright Holders:Copyright © 2017 Dietrich et al.
First Published:First published in mSphere 2(3):00090-17
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656551Arbovirus interactions with arthropod hostsAlain KohlMedical Research Council (MRC)MC_UU_12014/8MVLS III - CENTRE FOR VIRUS RESEARCH