The role of leucine in isoprenoid metabolism. Incorporation of [3-¹³C]leucine and of [2-³H,4-¹⁴C]-β,β-dimethylacrylic acid into phytosterols by tissue cultures of Andrographis paniculata

Anastasis, P., Freer, I. M., Overton, K. H., Rycroft, D. S. and Singh, S. B. (1985) The role of leucine in isoprenoid metabolism. Incorporation of [3-¹³C]leucine and of [2-³H,4-¹⁴C]-β,β-dimethylacrylic acid into phytosterols by tissue cultures of Andrographis paniculata. Journal of the Chemical Society: Chemical Communications, 1985(3), pp. 148-149. (doi: 10.1039/C39850000148)

Abstract

[3-¹³C]Leucine is incorporated into phytosterols by tissue cultures of Andrographis paniculata by breakdown to acetyl-CoA and its subsequent incorporation via (3S)-3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) and mevalonic acid; [2-³H,4-¹⁴C]-β,β-dimethylacrylic acid also is not incorporated intact.

• Item Type: Articles
• Keywords: Natural product chemistry, biosynthesis, NMR spectroscopy.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Rycroft, Dr David
• Authors: Anastasis, P., Freer, I. M., Overton, K. H., Rycroft, D. S., and Singh, S. B.
• Subjects: Q Science > QD Chemistry
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: Journal of the Chemical Society: Chemical Communications
• Publisher: Royal Society of Chemistry
• ISSN: 0022-4936