Heterogeneity of phenotype and function reflects the multistage development of T follicular helper cells

Trüb, M., Barr, T. A., Morrison, V. L. , Brown, S., Caserta, S., Rixon, J., Ivens, A. and Gray, D. (2017) Heterogeneity of phenotype and function reflects the multistage development of T follicular helper cells. Frontiers in Immunology, 8, 489. (doi:10.3389/fimmu.2017.00489) (PMID:28503175) (PMCID:PMC5408024)

[img]
Preview
Text
140206.pdf - Published Version
Available under License Creative Commons Attribution.

5MB

Abstract

T follicular helper cells (Tfh) provide crucial signals for germinal center (GC) formation, but Tfh populations are heterogeneous. While PD1hi Tfh are important in the GC response, the function of the PD1lo Tfh-like subset is unknown. We show that these cells, like the PD1hi GC–Tfh, depend upon B cells; however, their entry to follicles is independent of CXCR5 or cognate interactions with B cells. The differentiation into PD1hi Tfh is dependent on MHC class II interactions with B cells and requires CXCR5. Our data suggest a Tfh differentiation pathway that is initially B cell-independent, then dependent on non-cognate B cell interactions, and finally following cognate interaction with B cells and CXCR5-ligands allows the formation of GC–Tfh. The PD1lo Tfh-like cells make early cytokine responses and may represent precursors of CD4 memory cells.

Item Type:Articles
Additional Information:Funding Statement reads: This project was supported by the project grant form Wellcome Trust (094815) to DG. MT was in the receipt of an MRC Capacity Building PhD Studentship.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Morrison, Dr Vicky
Authors: Trüb, M., Barr, T. A., Morrison, V. L., Brown, S., Caserta, S., Rixon, J., Ivens, A., and Gray, D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Frontiers in Immunology
Publisher:Frontiers
ISSN:1664-3224
ISSN (Online):1664-3224
Copyright Holders:Copyright © 2017 Trüb, Barr, Morrison, Brown, Caserta, Rixon, Ivens and Gray
First Published:First published in Frontiers in Immunology 8: 489
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record