The anti-adhesive effect of curcumin on Candida albicans biofilms on denture materials

Alalwan, H., Rajendran, R., Lappin, D. F., Combet, E. , Shahzad, M., Robertson, D., Nile, C. J. , Williams, C. and Ramage, G. (2017) The anti-adhesive effect of curcumin on Candida albicans biofilms on denture materials. Frontiers in Microbiology, 8, 659. (doi:10.3389/fmicb.2017.00659) (PMID:28473808) (PMCID:PMC5397414)

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Abstract

The use of natural compounds as an alternative source of antimicrobials has become a necessity given the growing concern over global antimicrobial resistance. Polyphenols, found in various edible plants, offers one potential solution to this. We aimed to investigate the possibility of using curcumin within the context of oral health as a way of inhibiting and preventing the harmful development of Candida albicans biofilms. We undertook a series of adsorption experiments with varying concentrations of curcumin, showing that 50 ug/ml could prevent adhesion. This effect could be further synergised by the curcumin pretreatment of yeast cells to obtain significantly greater inhibition (>90, p<0.001). Investigation of the biological impact of curcumin showed that it preferentially affected immature morphological forms (yeast and germlings), and actively promoted aggregation of the cells. Transcriptional analyses showed that key adhesins were down-regulated (ALS1 and ALS3), whereas aggregation related genes (ALS5 and AAF1) were up-regulated. Collectively, these data demonstrated that curcumin elicits anti-adhesive effects and that induces transcription of genes integrally involved in the processes related to biofilm formation. Curcumin and associated polyphenols therefore have the capacity to be developed for use in oral healthcare to augment existing preventative strategies for candidal biofilms on the denture surface.

Item Type:Articles
Additional Information:HA is supported by the Iraqi Ministry of Higher Education and Scientific Research. RR is supported by the Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology 097377/Z/11/Z.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lappin, Dr David and Ramage, Professor Gordon and Rajendran, Dr Ranjith and ALALWAN, HASANAIN KAHTAN ABDULKHALIK and Combet Aspray, Dr Emilie and Robertson, Mr Douglas and Williams, Dr Craig and Nile, Dr Christopher
Authors: Alalwan, H., Rajendran, R., Lappin, D. F., Combet, E., Shahzad, M., Robertson, D., Nile, C. J., Williams, C., and Ramage, G.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Frontiers in Microbiology
Publisher:Frontiers
ISSN:1664-302X
ISSN (Online):1664-302X
Copyright Holders:Copyright © 2017 Alalwan, Rajendran, Lappin, Combet, Shahzad, Robertson, Nile, Williams and Ramage
First Published:First published in Frontiers in Microbiology 8:659
Publisher Policy:Reproduced under a Creative Commons License

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