Symmetry-breaking in branching epithelia: cells on micro-patterns under flow challenge the hypothesis of positive feedback by a secreted autocrine inhibitor of motility

Martin, K. C., Yuan, X., Stimac, G., Bannerman, K., Anderson, J., Roy, C., Glykofrydis, F., Yin, H. and Davies, J. A. (2017) Symmetry-breaking in branching epithelia: cells on micro-patterns under flow challenge the hypothesis of positive feedback by a secreted autocrine inhibitor of motility. Journal of Anatomy, 230(6), pp. 766-774. (doi:10.1111/joa.12599) (PMID:28369863)

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Abstract

Branching morphogenesis of epithelia involves division of cells into leader (tip) and follower (stalk) cells. Published work on cell lines in culture has suggested that symmetry-breaking takes place via a secreted autocrine inhibitor of motility, the inhibitor accumulating more in concave regions of the culture boundary, slowing advance of cells there, and less in convex areas, allowing advance and a further exaggeration of the concave/convex difference. Here we test this hypothesis using a two-dimensional culture system that includes strong flow conditions to remove accumulating diffusible secretions. We find that, while motility does indeed follow boundary curvature in this system, flow makes no difference: this challenges the hypothesis of control by a diffusible secreted autocrine inhibitor.

Item Type:Articles
Keywords:Autocrine secretion, branching morphogenesis, cell motility, positive feedback, self-organisation, shape.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Yin, Professor Huabing and Yuan, Dr Xiaofei
Authors: Martin, K. C., Yuan, X., Stimac, G., Bannerman, K., Anderson, J., Roy, C., Glykofrydis, F., Yin, H., and Davies, J. A.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of Anatomy
Publisher:Wiley
ISSN:0021-8782
ISSN (Online):1469-7580
Published Online:29 March 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Journal of Anatomy 230(6):766-774
Publisher Policy:Reproduced under a Creative Commons License

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