Russell, G.C., Grant, D.M., Lycett, S. , Bachofen, C., Caldow, G.L., Burr, P.D., Davie, K., Ambrose, N., Gunn, G.J. and Zadoks, R.N. (2017) Analysis of bovine viral diarrhoea virus: Biobank and sequence database to support eradication in Scotland. Veterinary Record, 180(18), 447. (doi: 10.1136/vr.104072) (PMID:28386029)
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Abstract
Samples from bovine viral diarrhoea virus (BVDV)—positive cattle were gathered by Scottish diagnostic laboratories and used to produce a Biobank of samples with associated location and identification data in support of the Scottish BVDV eradication scheme. The samples were subject to direct amplification and sequencing of the 5′-untranslated region (5′-UTR) to define the viral types and subtypes present. From 2693 samples collected prior to 2016, approximately 2300 sequences were obtained, representing 8 BVDV type 1 subtypes. No BVDV type 2 samples were detected. The samples came from all regions of the UK but 66 per cent were from Scotland. Analysis of the sequences showed great diversity in the 5′-UTR, with 1206 different sequences. Many samples carried virus with identical 5′-UTR sequences; often from single locations, but there were also examples of the same sequence being obtained from samples at several different locations. This work provides a resource that can be used to analyse the movement of BVDV strains both within Scotland and between Scotland and other nations, particularly in the latter stages of the Scottish eradication programme, and so inform the advice available to both livestock keepers and policymakers.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was funded by the Scottish Government Rural and Environment Science and Analytical Services Division (RESAS) as part of the EPIC Centre of Expertise on Animal Disease Outbreaks (www.epicscotland.org/; Twitter: @EpicScotland). |
| Keywords: | Pestivirus, eradication, livestock viruses, DNA sequences, genotypes. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Zadoks, Professor Ruth and Lycett, Dr Samantha |
| Authors: | Russell, G.C., Grant, D.M., Lycett, S., Bachofen, C., Caldow, G.L., Burr, P.D., Davie, K., Ambrose, N., Gunn, G.J., and Zadoks, R.N. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Veterinary Record |
| Publisher: | BMJ Publishing Group |
| ISSN: | 0042-4900 |
| ISSN (Online): | 2042-7670 |
| Published Online: | 06 April 2017 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Veterinary Record 180(18): 447 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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