A specific DIF binding protein in Dictyostelium

Insall, R. and Kay, R. R. (1990) A specific DIF binding protein in Dictyostelium. EMBO Journal, 9(10), pp. 3323-3328. (PMID:2170113) (PMCID:PMC552069)

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Differentiation Inducing Factor (DIF-1), a small chlorinated organic molecule which is produced during Dictyostelium development, is believed to be the morphogen that controls the stalk-specific pathway of differentiation. We describe the identification and characterization of a protease-sensitive activity from cell lysates which binds tritiated DIF-1 with the properties expected of a DIF receptor. Scatchard and linear subtraction plots show a single class of binding sites, of high affinity (Kd = 1.8 nM) and low abundance (1100 sites/cell). The activity elutes from heparin-agarose as a single peak. Various DIF-1 analogues compete for binding in proportion to their activities in a stalk cell differentiation bioassay. The amount of binding activity is developmentally regulated, peaking shortly before the appearance of the prestalk-prespore pattern and before the developmental rise in DIF concentration; the rise occurs at the same time that prestalk-specific genes become DIF inducible. Addition of cyclic AMP to aggregated cells, which induces post-aggregative gene expression in general, also induces the binding activity.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Insall, Professor Robert
Authors: Insall, R., and Kay, R. R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:EMBO Journal
Publisher:EMBO Press
ISSN (Online):1460-2075

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