Seven helix chemoattractant receptors transiently stimulate mitogen-activated protein kinase in Dictyostelium

Maeda, M., Aubry, L., Insall, R. , Gaskins, C., Devreotes, P. N. and Firtel, R. A. (1996) Seven helix chemoattractant receptors transiently stimulate mitogen-activated protein kinase in Dictyostelium. Journal of Biological Chemistry, 271(7), pp. 3351-3354. (doi:10.1074/jbc.271.7.3351) (PMID:8631932)

Full text not currently available from Enlighten.

Abstract

Mitogen-activated protein (MAP) kinases are involved in controlling a cell's responses to a variety of stimuli and can be activated by both protein tyrosine kinase and G protein-coupled receptors. It was shown previously that Dictyostelium MAP kinase ERK2 is required for normal activation of adenylyl cyclase and erk2 null cells are aggregation-deficient. In this manuscript, we show that the Dictyostelium MAP kinase ERK2 is rapidly and transiently activated in response to the chemoattractant cAMP. This response requires cAMP receptors, but is independent of the coupled Gα2 subunit and the only known Gβ subunit. These data indicate that ligand-mediated receptor activation of adenylyl cyclase requires two receptor-dependent pathways, one of which requires heterotrimeric G proteins, including Gα2 and the only known Gβ subunit, and the second of which requires ERK2. Our results suggest that ERK2 may be activated by a novel receptor-mediated pathway.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Insall, Professor Robert
Authors: Maeda, M., Aubry, L., Insall, R., Gaskins, C., Devreotes, P. N., and Firtel, R. A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Journal of Biological Chemistry
Publisher:American Society for Biochemistry and Molecular Biology, Inc.
ISSN:0021-9258
ISSN (Online):1083-351X

University Staff: Request a correction | Enlighten Editors: Update this record