Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation

Periz, J. et al. (2017) Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation. eLife, 6, e24119. (doi:10.7554/eLife.24119) (PMID:28322189) (PMCID:PMC5375643)

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Abstract

Apicomplexan actin is important during the parasite's life cycle. Its polymerization kinetics are unusual, permitting only short, unstable F-actin filaments. It has not been possible to study actin in vivo and so its physiological roles have remained obscure, leading to models distinct from conventional actin behaviour. Here a modified version of the commercially available actin-chromobody was tested as a novel tool for visualising F-actin dynamics in Toxoplasma gondii. Cb labels filamentous actin structures within the parasite cytosol and labels an extensive F-actin network that connects parasites within the parasitophorous vacuole and allows vesicles to be exchanged between parasites. In the absence of actin, parasites lack a residual body and inter-parasite connections and grow in an asynchronous and disorganized manner. Collectively, these data identify new roles for actin in the intracellular phase of the parasites lytic cycle and provide a robust new tool for imaging parasitic F-actin dynamics.

Item Type:Articles
Keywords:Apicomplexa, Toxoplasma, actin, cell biology, infectious disease, microbiology, nanobodies, replication
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Periz, Dr Javier and Reimer, Ms Madita and Harding, Dr Clare and Whitelaw, Mr Jamie and Gras, Dr Simon and Insall, Professor Robert and Lemgruber Soares, Dr Leandro
Authors: Periz, J., Whitelaw, J., Harding, C., Gras, S., Del Rosario Minina, M. I., Latorre-Barragan, F., Lemgruber Soares, L., Reimer, M. A., Insall, R., Heaslip, A., and Meissner, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:eLife
Publisher:eLife Sciences Publications
ISSN:2050-084X
ISSN (Online):2050-084X
Copyright Holders:Copyright © 2017 Periz et al.
First Published:First published in eLife 6:e24119
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
501601Systematic analysis of essential parasite genes linked to invasion of the host cell in Toxoplasma gondiiMarkus MeissnerWellcome Trust (WELLCOME)087582/Z/08/ZIII - PARASITOLOGY