Cell motility and SCAR localisation in axenically growing Dictyostelium cells

Pollitt, A. Y., Blagg, S. L., Ibarra, N. and Insall, R. H. (2006) Cell motility and SCAR localisation in axenically growing Dictyostelium cells. European Journal of Cell Biology, 85(9-10), pp. 1091-1098. (doi:10.1016/j.ejcb.2006.05.014) (PMID:16822579)

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Abstract

Dictyostelium is a popular experimental organism, in particular for studies of actin dynamics, cell motility and chemotaxis. We find that the motility of axenic cells is unexpectedly different from other strains during growth. In particular, vegetative AX3 cells do not show detectable localisation of SCAR and its regulatory complex to actin-rich protrusions such as filopodia and pseudopodia. Similarly, a range of different mutations, in particular knockouts of members of the SCAR complex and Ras proteins, cause different phenotypes during vegetative growth in different parental strains. Development reverses this unusual behaviour; aggregation-competent AX3 cells localise SCAR in the same way as cells of other strains and species. Studies on cell motility using vegetative cells should therefore be interpreted with caution.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Insall, Professor Robert
Authors: Pollitt, A. Y., Blagg, S. L., Ibarra, N., and Insall, R. H.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:European Journal of Cell Biology
Publisher:Elsevier
ISSN:0171-9335
ISSN (Online):1618-1298
Published Online:05 July 2006

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