Hecht, L., Barron, L.D., Gargaro, A.R., Wen, Z.Q. and Hug, W. (1992) Raman optical activity instrument for biochemical studies. Journal of Raman Spectroscopy, 23(7), pp. 401-411. (doi: 10.1002/jrs.1250230706)
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Abstract
A simple and fully computer-controlled backscattering incident circular polarization Raman optical activity (ICP ROA) instrument suitable for chioptic studies of biologically significant molecules in aqueous solution is described. It consists of a fast single-stage monochromator equipped with a holographic edge filter, a highly efficient holographic difiaction grating and a thinned back illuminated thermoelectrically cooled charge-coupled device detector as the key spectral elements. A longitudinal electro-optic modulator is employed to switch between orthogonal circular polarization states in the incident laser radiation within a backscattering geometry. A thick Lyot depolarizer is used for the depolarization of the backscattered Raman radiation. High-quality backscattering ICP ROA spectra of (1S)-( − )-trans-pinane, (1R)-( + )-trans-pinane, L-alanine, L-alanyl-L-alanine, lysozyme, D-glucose, α-D-cyclodextrin and 2′-deoxycytidine are presented as typical examples of the excellent performance characteristics.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Hecht, Dr Lutz and Barron, Professor Laurence |
| Authors: | Hecht, L., Barron, L.D., Gargaro, A.R., Wen, Z.Q., and Hug, W. |
| College/School: | College of Science and Engineering > School of Chemistry |
| Journal Name: | Journal of Raman Spectroscopy |
| Publisher: | Wiley |
| ISSN: | 0377-0486 |
| ISSN (Online): | 1097-4555 |
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