Raman optical activity instrument for biochemical studies

Hecht, L., Barron, L.D., Gargaro, A.R., Wen, Z.Q. and Hug, W. (1992) Raman optical activity instrument for biochemical studies. Journal of Raman Spectroscopy, 23(7), pp. 401-411. (doi: 10.1002/jrs.1250230706)

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A simple and fully computer-controlled backscattering incident circular polarization Raman optical activity (ICP ROA) instrument suitable for chioptic studies of biologically significant molecules in aqueous solution is described. It consists of a fast single-stage monochromator equipped with a holographic edge filter, a highly efficient holographic difiaction grating and a thinned back illuminated thermoelectrically cooled charge-coupled device detector as the key spectral elements. A longitudinal electro-optic modulator is employed to switch between orthogonal circular polarization states in the incident laser radiation within a backscattering geometry. A thick Lyot depolarizer is used for the depolarization of the backscattered Raman radiation. High-quality backscattering ICP ROA spectra of (1S)-( − )-trans-pinane, (1R)-( + )-trans-pinane, L-alanine, L-alanyl-L-alanine, lysozyme, D-glucose, α-D-cyclodextrin and 2′-deoxycytidine are presented as typical examples of the excellent performance characteristics.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Hecht, Dr Lutz and Barron, Professor Laurence
Authors: Hecht, L., Barron, L.D., Gargaro, A.R., Wen, Z.Q., and Hug, W.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Journal of Raman Spectroscopy
ISSN (Online):1097-4555

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