Magnetically levitated mesenchymal stem cell spheroids cultured with a collagen gel maintain phenotype and quiescence

Lewis, N. S., Lewis, E. E.L., Mullin, M., Wheadon, H. , Dalby, M. J. and Berry, C. C. (2017) Magnetically levitated mesenchymal stem cell spheroids cultured with a collagen gel maintain phenotype and quiescence. Journal of Tissue Engineering, 8, pp. 1-11. (doi:10.1177/2041731417704428) (PMID:28616152) (PMCID:PMC5460809)

[img]
Preview
Text
138644.pdf - Published Version
Available under License Creative Commons Attribution.

880kB

Abstract

Multicellular spheroids are an established system for three-dimensional cell culture. Spheroids are typically generated using hanging drop or non-adherent culture; however, an emerging technique is to use magnetic levitation. Herein, mesenchymal stem cell spheroids were generated using magnetic nanoparticles and subsequently cultured within a type I collagen gel, with a view towards developing a bone marrow niche environment. Cells were loaded with magnetic nanoparticles, and suspended beneath an external magnet, inducing self-assembly of multicellular spheroids. Cells in spheroids were viable and compared to corresponding monolayer controls, maintained stem cell phenotype and were quiescent. Interestingly, core spheroid necrosis was not observed, even with increasing spheroid size, in contrast to other commonly used spheroid systems. This mesenchymal stem cell spheroid culture presents a potential platform for modelling in vitro bone marrow stem cell niches, elucidating interactions between cells, as well as a useful model for drug delivery studies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lewis, Natasha and Berry, Dr Catherine and Dalby, Professor Matthew and Wheadon, Dr Helen and Mullin, Mrs Margaret
Authors: Lewis, N. S., Lewis, E. E.L., Mullin, M., Wheadon, H., Dalby, M. J., and Berry, C. C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of Tissue Engineering
Publisher:SAGE
ISSN:2041-7314
ISSN (Online):2041-7314
Published Online:24 April 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Journal of Tissue Engineering 8:1-11
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
5887510BBSRC Doctoral Training Partnership 2012George BaillieBiotechnology and Biological Sciences Research Council (BBSRC)BB/J013854/1MVLS COLLEGE SENIOR MANAGEMENT