Gossel, G., Hogan, T., Cownden, D., Seddon, B. and Yates, A. J. (2017) Memory CD4 T cell subsets are kinetically heterogeneous and replenished from naive T cells at high levels. eLife, 6, e23013. (doi: 10.7554/eLife.23013) (PMID:28282024) (PMCID:PMC5426903)
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Abstract
Characterising the longevity of immunological memory requires establishing the rules underlying the renewal and death of peripheral T cells. However, we lack knowledge of the population structure and how self-renewal and de novo influx contribute to maintenance of memory compartments. Here, we characterise the kinetics and structure of murine CD4 T cell memory subsets by measuring the rates of influx of new cells and using detailed timecourses of DNA labelling that also distinguish the behaviour of recently divided and quiescent cells. We find that both effector and central memory CD4 T cells comprise subpopulations with highly divergent rates of turnover, and show that inflows of new cells sourced from the naive pool strongly impact estimates of memory cell lifetimes and division rates. We also demonstrate that the maintenance of CD4 T cell memory subsets in healthy mice is unexpectedly and strikingly reliant on this replenishment.
Item Type: | Articles |
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Keywords: | Computational biology, immunology, mouse, systems biology. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Cownden, Dr Daniel and Yates, Professor Andrew and Gossel, Dr Graeme |
Authors: | Gossel, G., Hogan, T., Cownden, D., Seddon, B., and Yates, A. J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | eLife |
Publisher: | eLife Sciences Publications |
ISSN: | 2050-084X |
ISSN (Online): | 2050-084X |
Copyright Holders: | Copyright © 2017 Grossel et al. |
First Published: | First published in eLife 6: e23013 |
Publisher Policy: | Reproduced under a Creative Commons license |
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