Exploration of haplotype research consortium imputation for genome-wide association studies in 20,032 Generation Scotland participants

Nagy, R. et al. (2017) Exploration of haplotype research consortium imputation for genome-wide association studies in 20,032 Generation Scotland participants. Genome Medicine, 9, 23. (doi: 10.1186/s13073-017-0414-4) (PMID:28270201) (PMCID:PMC5339960)

[img]
Preview
Text
138515.pdf - Published Version
Available under License Creative Commons Attribution.

1MB

Abstract

Background: The Generation Scotland: Scottish Family Health Study (GS:SFHS) is a family-based population cohort with DNA, biological samples, socio-demographic, psychological and clinical data from approximately 24,000 adult volunteers across Scotland. Although data collection was cross-sectional, GS:SFHS became a prospective cohort due to of the ability to link to routine Electronic Health Record (EHR) data. Over 20,000 participants were selected for genotyping using a large genome-wide array. Methods: GS:SFHS was analysed using genome-wide association studies (GWAS) to test the effects of a large spectrum of variants, imputed using the Haplotype Research Consortium (HRC) dataset, on medically relevant traits measured directly or obtained from EHRs. The HRC dataset is the largest available haplotype reference panel for imputation of variants in populations of European ancestry and allows investigation of variants with low minor allele frequencies within the entire GS:SFHS genotyped cohort. Results: Genome-wide associations were run on 20,032 individuals using both genotyped and HRC imputed data. We present results for a range of well-studied quantitative traits obtained from clinic visits and for serum urate measures obtained from data linkage to EHRs collected by the Scottish National Health Service. Results replicated known associations and additionally reveal novel findings, mainly with rare variants, validating the use of the HRC imputation panel. For example, we identified two new associations with fasting glucose at variants near to Y_RNA and WDR4 and four new associations with heart rate at SNPs within CSMD1 and ASPH, upstream of HTR1F and between PROKR2 and GPCPD1. All were driven by rare variants (minor allele frequencies in the range of 0.08–1%). Proof of principle for use of EHRs was verification of the highly significant association of urate levels with the well-established urate transporter SLC2A9.

Item Type:Articles
Keywords:Electronic health records, genetics, genome-wide association studies (GWAS), glucose, Haplotype Research Consortium (HRC), heart rate, imputation, quantitative trait, urate.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Padmanabhan, Professor Sandosh
Authors: Nagy, R., Boutin, T. S., Marten, J., Huffman, J. E., Kerr, S. M., Campbell, A., Evenden, L., Gibson, J., Amador, C., Howard, D. M., Navarro, P., Morris, A., Deary, I. J., Hocking, L. J., Padmanabhan, S., Smith, B. H., Joshi, P., Wilson, J. F., Hastie, N. D., Wright, A. F., McIntosh, A. M., Porteous, D. J., Haley, C. S., Vitart, V., and Hayward, C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Genome Medicine
Publisher:BioMed Central
ISSN:1756-994X
ISSN (Online):1756-994X
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Genome Medicine 9: 23
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
381721Generation ScotlandAnna DominiczakScottish Executive Health Department (SEHHD-CSO)CZD/16/6RI CARDIOVASCULAR & MEDICAL SCIENCES