Determination of enantiomeric excess using Raman optical activity

Hecht, L., Phillips, A. L. and Barron, L. D. (1995) Determination of enantiomeric excess using Raman optical activity. Journal of Raman Spectroscopy, 26(8-9), pp. 727-732. (doi: 10.1002/jrs.1250260820)

Abstract

A simple method for the determination of enantiomeric excess is described. It is based on depolarized Raman optical activity (ROA) measurements in right-angle scattering employing the incident ciruclar polarization (ICP) modulation approach. The accuracy of this method was assessed utilizing α-pinene as a chiral test compound. The four α-pinene ICP ROA signals associated with the Raman bands observed at ca. 772, 787, 887 and 929 cm−1 were selected to establish a linear relationship between ROA signal strength and enantiomeric composition for a set of calibration mixtures containing both α-pinene enantiomers. Using this relationship, a (+)-α-pinene sample content of 16.3% was determined for a blind mixture containing 83.6% (1S)-( — )-α-pinene and 16.4% (1R)-(+)-α-pinene. Similary, utilizing additional spectral subtraction procedures, a (+)-α-pinene sample content of 1.5% was estimated for a test mixture consisting of 50.1% (1S)-( — )-α-pinene and 49.9% (1S)-( — )-trans-pinane.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Hecht, Dr Lutz and Barron, Professor Laurence
• Authors: Hecht, L., Phillips, A. L., and Barron, L. D.
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: Journal of Raman Spectroscopy
• Publisher: Wiley
• ISSN: 0377-0486
• ISSN (Online): 1097-4555