Replication-deficient particles: new insights into the next generation of bluetongue virus vaccines

Celma, C. C. et al. (2017) Replication-deficient particles: new insights into the next generation of bluetongue virus vaccines. Journal of Virology, 91(1), e01892-16. (doi: 10.1128/jvi.01892-16) (PMID:27795442) (PMCID:PMC5165199)

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Abstract

Bluetongue virus (BTV) is endemic in many parts of the world, often causing severe hemorrhagic disease in livestock. To date, at least 27 different serotypes have been recognized. Vaccination against all serotypes is necessary to protect susceptible animals and to prevent onward spread of the virus by insect vectors. In our previous studies, we generated replication-deficient (disabled infectious single-cycle [DISC]) virus strains for a number of serotypes and reported preliminary data on their protective efficacy in animals. In this report, to advance the DISC vaccines to the marketplace, we investigated different parameters of these DISC vaccines. First, we demonstrated the genetic stabilities of these vaccine strains and also the complementing cell line. Subsequently, the optimal storage conditions of vaccines, including additives, temperature, and desiccation, were determined and their protective efficacies in animals confirmed. Furthermore, to test if mixtures of different vaccine strains could be tolerated, we tested cocktails of DISC vaccines in combinations of three or six different serotypes in sheep and cattle, the two natural hosts of BTV. Groups of sheep vaccinated with a cocktail of six different vaccines were completely protected from challenge with individual virulent serotypes, both in early challenge and after 5 months of challenge without any clinical disease. There was no interference in protection between the different vaccines. Protection was also achieved in cattle with a mixture of three vaccine strains, albeit at a lesser level than in sheep. Our data support and validate the suitability of these virus strains as the next-generation vaccines for BTV.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stewart, Dr Meredith
Authors: Celma, C. C., Stewart, M., Wernike, K., Eschbaumer, M., Gonzalez-Molleda, L., Breard, E., Schulz, C., Hoffmann, B., Haegeman, A., De Clercq, K., Zientara, S., van Rijn, P. A., Beer, M., Roy, P., and Perlman, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Virology
Publisher:American Society for Microbiology
ISSN:0022-538X
ISSN (Online):1098-5514
Published Online:26 October 2016
Copyright Holders:Copyright © 2016 Celma et al.
First Published:First published in Journal of Virology 91(1):e01892-16
Publisher Policy:Reproduced under a Creative Commons License

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