A new perspective on protein structure and dynamics from Raman optical activity

Barron, L.D., Wilson, G., Hecht, L. and Blanch, E.W. (1997) A new perspective on protein structure and dynamics from Raman optical activity. In: Carmona, P., Navarro, R. and Hernanz, A. (eds.) Spectroscopy of Biological Molecules: Modern Trends. Springer Netherlands, pp. 9-10. ISBN 9789401063692 (doi:10.1007/978-94-011-5622-6_109)

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The vlue of vibrational spectroscopy in the study of biopolymers has been greatly enhanced by adding the new dimension of optical activity [1]. The Raman optical activity (ROA) approach, which measures a small difference in the intensity of Raman scattering in right and left circularly polarized incident light, has been transformed by recent technical advances into a powerful new biochemical spectroscopy [2]. Since protein ROA spectra contains bands from loops and turns in addition to secondary structure, they contain information about the tertiary backbone fold and hence the three-dimensional solution structure. This, plus a special sensitivity to conformational mobility and the short timescale of the Raman scattering process, makes ROA ideal for studying dynamic aspects of structure in both non-native [3, 4] and native [5] states.

Item Type:Book Sections
Glasgow Author(s) Enlighten ID:Hecht, Dr Lutz and Barron, Professor Laurence
Authors: Barron, L.D., Wilson, G., Hecht, L., and Blanch, E.W.
College/School:College of Science and Engineering > School of Chemistry
Publisher:Springer Netherlands

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