Candida albicans biofilm heterogeneity does not influence denture stomatitis but strongly influences denture cleansing capacity

O'Donnell, L. E., Alalwan, H. K. A., Kean, R., Calvert, G., Nile, C. J. , Lappin, D. J., Robertson, D. , Williams, C., Ramage, G. and Sherry, L. (2017) Candida albicans biofilm heterogeneity does not influence denture stomatitis but strongly influences denture cleansing capacity. Journal of Medical Microbiology, 66(1), pp. 54-60. (doi:10.1099/jmm.0.000419) (PMID:28032543)

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Abstract

Approximately 20  % of the UK population wear some form of denture prosthesis, resulting in denture stomatitis in half of these individuals. Candida albicans is primarily attributed as the causative agent, due to its biofilm -forming ability. Recently, there has been increasing evidence of C. albicans biofilm heterogeneity and the negative impact it can have clinically; however, this phenomenon has yet to be studied in relation to denture isolates. The aims of this study were to evaluate C. albicans biofilm formation of clinical denture isolates in a denture environment and to assess antimicrobial activity of common denture cleansers against these tenacious communities. C. albicans isolated from dentures of healthy and diseased individuals was quantified using real-time PCR and biofilm biomass assessed using crystal violet. Biofilm development on the denture substratum poly(methyl methacrylate), Molloplast B and Ufi-gel was determined. Biofilm formation was assessed using metabolic and biomass stains, following treatment with denture hygiene products. Although C. albicans was detected in greater quantities in diseased individuals, it was not associated with increased biofilm biomass. Denture substrata were shown to influence biofilm biomass, with poly(methyl methacrylate) providing the most suitable environment for C. albicans to reside. Of all denture hygiene products tested, Milton had the most effective antimicrobial activity, reducing biofilm biomass and viability the greatest. Overall, our results highlight the complex nature of denture- related disease, and disease development cannot always be attributed to a sole cause. It is the distinct combination of various factors that ultimately determines the pathogenic outcome.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lappin, Dr David and Kean, Mr Ryan and Calvert, Mr Gareth and Robertson, Mr Douglas and Ramage, Professor Gordon and Williams, Dr Craig and Sherry, Dr Leighann and Nile, Dr Chris and ALALWAN, HASANAIN KAHTAN ABDULKHALIK and O'Donnell, Dr Lindsay
Authors: O'Donnell, L. E., Alalwan, H. K. A., Kean, R., Calvert, G., Nile, C. J., Lappin, D. J., Robertson, D., Williams, C., Ramage, G., and Sherry, L.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Journal of Medical Microbiology
Publisher:Microbiology Society
ISSN:0022-2615
ISSN (Online):1473-5644
Published Online:06 February 2017
Copyright Holders:Copyright © 2017 Microbiology Society
First Published:First published in Journal of Medical Microbiology 66(1):54-60
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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