Sequence analysis of mRNA transcripts encoding Jembrana disease virus Tat-1 in vivo

Setiyaningsih, S., Desport, M., Stewart, M. E., Hartaningsih, N. and Wilcox, G. E. (2008) Sequence analysis of mRNA transcripts encoding Jembrana disease virus Tat-1 in vivo. Virus Research, 132(1-2), pp. 220-225. (doi: 10.1016/j.virusres.2007.11.004) (PMID:18096262)

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Abstract

Jembrana disease virus (JDV) is a lentivirus which replicates to very high titres in vivo and its Tat-1 protein has been shown to be a potent transactivator in vitro. Analysis of tat mRNA transcripts produced early in infection studies identified four predominant species which were generated by multiple splicing events. The use of a splice donor downstream of tat-1 was common indicating that a Tat-1 protein of 97 amino acids is expressed during the acute phase of Jembrana disease. The presence of an in-frame stop codon between tat-1 and tat-2 was identified in transcripts from three different strains of JDV confirming that expression of a single exon Tat-1 correlates with high viral titres in vivo. Sequence conservation in the regions of tat-1 that are critical for RNA binding and transcription activation in three different virus strains was high and the tat-2 sequences were completely conserved.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stewart, Dr Meredith
Authors: Setiyaningsih, S., Desport, M., Stewart, M. E., Hartaningsih, N., and Wilcox, G. E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Virus Research
Publisher:Elsevier
ISSN:0168-1702
Published Online:21 December 2007

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