Analysis of Jembrana disease virus mRNA transcripts produced during acute infection demonstrates a complex transcription pattern

Stewart, M. E., Desport, M., Setiyaningsih, S., Hartaningsih, N. and Wilcox, G. E. (2008) Analysis of Jembrana disease virus mRNA transcripts produced during acute infection demonstrates a complex transcription pattern. Virus Research, 135(2), pp. 336-339. (doi: 10.1016/j.virusres.2008.03.017) (PMID:18466992)

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Abstract

Jembrana disease virus (JDV) is an unusual bovine lentivirus that causes an acute disease syndrome with a 20% case fatality rate after a short incubation period in Bos javanicus (Bali cattle) in Indonesia. Analysis of tat mRNA transcription patterns has identified up to six differently spliced transcripts indicating that, in common with other lentiviruses, JDV uses a complex splicing pattern. RT-PCR analysis of mRNA transcripts produced during the acute phase of infection with JDVTAB/87 revealed at least 12 differently spliced transcripts involving 9 different splice sites. A single unspliced gag/pol transcript, singly spliced vif and tmx specific transcripts and alternatively spliced env, tat and rev transcripts were identified. A 67 nucleotide putative non-coding exon was identified that shared the same splice acceptor (SA) as vif and was incorporated into alternative transcripts of tat, rev and env.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stewart, Dr Meredith
Authors: Stewart, M. E., Desport, M., Setiyaningsih, S., Hartaningsih, N., and Wilcox, G. E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Virus Research
Publisher:Elsevier
ISSN:0168-1702
ISSN (Online):1872-7492
Published Online:07 May 2008

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