Time-lapse imaging of glial-axonal interactions

Ioannidou, K., Edgar, J. M. and Barnett, S. C. (2015) Time-lapse imaging of glial-axonal interactions. Current Protocols in Neuroscience, 72, 2.23.1-2.23.14. (doi: 10.1002/0471142301.ns0223s72) (PMID:26131661)

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Abstract

In the central nervous system (CNS), myelin is formed by oligodendrocytes that are derived from precursor cells, known as oligodendrocyte precursor cells (OPCs). Successive stages of OPC interactions with the axons can be visualized in vitro and ex vivo using mixed neural cell cultures and pieces of intact spinal cord, respectively. OPCs and their differentiation can be imaged using cell-type-specific markers or green fluorescent protein (GFP) tags. This protocol describes methodology for generating these two systems for time-lapse imaging of dynamic cell interactions using fluorescent and 2-photon microscopy.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ioannidou, Dr Kalliopi and Barnett, Professor Susan
Authors: Ioannidou, K., Edgar, J. M., and Barnett, S. C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Current Protocols in Neuroscience
Publisher:Wiley
ISSN:1934-8584
ISSN (Online):1934-8576
Published Online:01 July 2015

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