Surface acoustic waves induced micropatterning of cells in gelatin methacryloyl (GelMA) hydrogels

Naseer, S. M. et al. (2017) Surface acoustic waves induced micropatterning of cells in gelatin methacryloyl (GelMA) hydrogels. Biofabrication, 9(1), 015020. (doi:10.1088/1758-5090/aa585e) (PMID:28195834)

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Abstract

Acoustic force patterning is an emerging technology that provides a platform to control the spatial location of cells in a rapid, accurate, yet contactless manner. However, very few studies have been reported on the usage of acoustic force patterning for the rapid arrangement of biological objects, such as cells, in a three-dimensional (3D) environment. In this study, we report on a bio-acoustic force patterning technique, which uses surface acoustic waves (SAWs) for the rapid arrangement of cells within an extracellular matrix-based hydrogel such as gelatin methacryloyl (GelMA). A proof-of-principle was achieved through both simulations and experiments based on the in-house fabricated piezoelectric SAW transducers, which enabled us to explore the effects of various parameters on the performance of the built construct. The SAWs were applied in a fashion that generated standing SAWs (SSAWs) on the substrate, the energy of which subsequently was transferred into the gel, creating a rapid, and contactless alignment of the cells (<10 s, based on the experimental conditions). Following ultraviolet radiation induced photo-crosslinking of the cell encapsulated GelMA pre-polymer solution, the patterned cardiac cells readily spread after alignment in the GelMA hydrogel and demonstrated beating activity in 5–7 days. The described acoustic force assembly method can be utilized not only to control the spatial distribution of the cells inside a 3D construct, but can also preserve the viability and functionality of the patterned cells (e.g. beating rates of cardiac cells). This platform can be potentially employed in a diverse range of applications, whether it is for tissue engineering, in vitro cell studies, or creating 3D biomimetic tissue structures.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Naseer, Mr Shahid Mohammad and Cooper, Professor Jonathan
Authors: Naseer, S. M., Manbachi, A., Samandari, M., Walch, P., Gao, Y., Zhang, Y. S., Davoudi, F., Wang, W., Abrinia, K., Cooper, J. M., Khademhosseini, A., and Shin, S. R.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Biofabrication
Publisher:IOP Publishing
ISSN:1758-5082
ISSN (Online):1758-5090
Published Online:13 February 2017
Copyright Holders:Copyright © 2017 IOP Publishing Ltd
First Published:First published in Biofabrication 9(1):015020
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING