Candida albicans mycofilms support Staphylococcus aureus colonization and enhances miconazole resistance in dual-species interactions

Kean, R. et al. (2017) Candida albicans mycofilms support Staphylococcus aureus colonization and enhances miconazole resistance in dual-species interactions. Frontiers in Microbiology, 8, 258. (doi:10.3389/fmicb.2017.00258)

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Publisher's URL: http://journal.frontiersin.org/article/10.3389/fmicb.2017.00258

Abstract

Polymicrobial inter-kingdom biofilm infections represent a clinical management conundrum. The presence of co-isolation of bacteria and fungi complicates the ability to routinely administer single antimicrobial regimens, and synergy between the microorganisms influences infection severity. We therefore investigated the nosocomial pathogens Staphylococcus aureus and Candida albicans with respect to antimicrobial intervention. We characterised the interaction using biofilm assays and evaluated the effect of miconazole treatment using in vitro and in vivo assays. Finally, we assessed the impact of biofilm extracellular matrix (ECM) on these interactions. Data indicated that the C. albicans mycofilms supported adhesion and colonisation by S. aureus through close interactions with hyphal elements, significantly increasing S. aureus biofilm formation throughout biofilm maturation. Miconazole sensitivity was shown to be reduced in both mono- and dual-species biofilms compared to planktonic cells. Within a three-dimensional biofilm model sensitivity was also hindered. Galleria mellonella survival analysis showed both enhanced pathogenicity of the dual-species infection, which was concomitantly desensitised to miconazole treatment. Analysis of the ECM revealed the importance of extracellular DNA (eDNA), which supported the adhesion of S. aureus and the development of the dual-species biofilm structures. Collectively, these data highlight the clinical importance of dual-species inter-kingdom biofilm infections, though also provides translational opportunities to manage them more effectively.

Item Type:Articles
Additional Information:RR is supported by the Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology 097377/Z/11/Z.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burgess, Dr Karl and Kean, Mr Ryan and Haggarty, Ms Jennifer and Ramage, Professor Gordon and Rajendran, Dr Ranjith and Townsend, Miss Eleanor
Authors: Kean, R., Rajendran, R., Haggarty, J., Townsend, E. M., Short, B., Burgess, K. E., Lang, S., Millington, O., Mackay, W., Williams, C., and Ramage, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Frontiers in Microbiology
Publisher:Frontiers
ISSN:1664-302X
ISSN (Online):1664-302X
Copyright Holders:Copyright © 2017 Kean, Rajendran, Haggarty, Townsend, Short, Burgess, Lang, Millington, Mackay, Williams and Ramage
First Published:First published in Frontiers in Microbiology 8: 258
Publisher Policy:Reproduced under a Creative Commons License

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