Wolbachia modulates lipid metabolism in Aedes albopictus mosquito cells

Molloy, J. C., Sommer, U., Viant, M. R. and Sinkins, S. P. (2016) Wolbachia modulates lipid metabolism in Aedes albopictus mosquito cells. Applied and Environmental Microbiology, 82(10), pp. 3109-3120. (doi:10.1128/AEM.00275-16) (PMID:26994075) (PMCID:PMC4959074)

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Abstract

Certain strains of the intracellular endosymbiont Wolbachia can strongly inhibit or block the transmission of viruses such as dengue virus (DENV) by Aedes mosquitoes, and the mechanisms responsible are still not well understood. Direct infusion and liquid chromatography-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry-based lipidomics analyses were conducted using Aedes albopictus Aa23 cells that were infected with the wMel and wMelPop strains of Wolbachia in comparison to uninfected Aa23-T cells. Substantial shifts in the cellular lipid profile were apparent in the presence of Wolbachia. Most significantly, almost all sphingolipid classes were depleted, and some reductions in diacylglycerols and phosphatidylcholines were also observed. These lipid classes have previously been shown to be selectively enriched in DENV-infected mosquito cells, suggesting that Wolbachia may produce a cellular lipid environment that is antagonistic to viral replication. The data improve our understanding of the intracellular interactions between Wolbachia and mosquitoes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sinkins, Professor Steven
Authors: Molloy, J. C., Sommer, U., Viant, M. R., and Sinkins, S. P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Applied and Environmental Microbiology
Publisher:American Society for Microbiology
ISSN:0099-2240
ISSN (Online):1098-5336
Copyright Holders:Copyright © 2016 Molloy et al.
First Published:First published in Applied and Environmental Microbiology 82(10):3109-3120
Publisher Policy:Reproduced under a creative commons license

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