Barnett, S. C. and Evans, C. H. (1990) Influence of extracellular calcium on cell permeabilization and growth regulation by the lymphokine leukoregulin. Journal of Cellular Biochemistry, 43(1), pp. 89-101. (doi: 10.1002/jcb.240430109) (PMID:2112134)
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Abstract
Permeablization of human K562 leukemia cells was measured in the presence and absence of extracellular ionic calcium to examine the relationship of ionic calcium to increased membrane permeability and the inhibition of cell proliferation by this lymphokine. In the absence of extracellular calcium, the ability of leukoregulin to permeabilize the cell membrane is diminished but is fully restored by addition of 1mM extracellular Ca++ as shown flow cytometrically by loss of intracellular fluorescien. Membrane permeability is also increased by calcium ionophore A23187 but permeablization is completely blocked in calcium-free medium despite the intramembrane presence of the calcium ionophore. Membrane permeablization by the lectin phytohemagglutinin, in contrast, is independent of extracellular calucium. A similar divergence in cell proliferation activity of the three modulators of calucium flux and membrane permeability occurs in the absence of extracellular calucium. Leukoregulin inhibition of cell proliferation is abolished, inhibition by calucium ionophore A23817 is greatly reduced, and inhibition by phytohemagglutinin in unchanged. Leukoregulin permeabilized K562 cells isolated by fluorescence activated cell storing resume proliferation after 72 h. In contracts cells permeablized by calucium inonphore A23187 or phytohemagglutinin fail to resume proliferation by 7 days. The membrane permeablizing action of leukoregulin is, therefore, partially dependent upon extracellular calcium. It is also effected through a mechanism other than ionophore transport or lectin type tansmembrane signalling, and is accompanied by a reversible inhibition of cell proliferation.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Barnett, Professor Susan |
| Authors: | Barnett, S. C., and Evans, C. H. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Journal of Cellular Biochemistry |
| Publisher: | Wiley |
| ISSN: | 0730-2312 |
| ISSN (Online): | 1097-4644 |
| Published Online: | 19 February 2004 |
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