Mechanistic modeling of a rewritable recombinase addressable data module

Bowyer, J., Zhao, J., Subsoontorn, P., Wong, W., Rosser, S. and Bates, D. (2016) Mechanistic modeling of a rewritable recombinase addressable data module. IEEE Transactions on Biomedical Circuits and Systems, 10(6), pp. 1161-1170. (doi:10.1109/tbcas.2016.2526668) (PMID:27244749)

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Abstract

Many of the most important applications predicted to arise from Synthetic Biology will require engineered cellular memory with the capability to store data in a rewritable and reversible manner upon induction by transient stimuli. DNA recombination provides an ideal platform for cellular data storage and has allowed the development of a rewritable recombinase addressable data (RAD) module, capable of efficient data storage within a chromosome. Here, we develop the first detailed mechanistic model of DNA recombination, and validate it against a new set of in vitro data on recombination efficiencies across a range of different concentrations of integrase and gp3. Investigation of in vivo recombination dynamics using our model reveals the importance of fully accounting for all mechanistic features of DNA recombination in order to accurately predict the effect of different switching strategies on RAD module performance, and highlights its usefulness as a design tool for building future synthetic circuitry.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Rosser, Professor Susan and Zhao, Miss Jia
Authors: Bowyer, J., Zhao, J., Subsoontorn, P., Wong, W., Rosser, S., and Bates, D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:IEEE Transactions on Biomedical Circuits and Systems
Publisher:IEEE
ISSN:1932-4545
ISSN (Online):1940-9990
Published Online:24 May 2016
Copyright Holders:Copyright © 2016 IEEE
First Published:First published in IEEE Transactions on Biomedical Circuits and Systems 10(6):1161-1170
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
519111Sandpit: Synthetic integrons for continuous directed evolution of complex genetic ensemblesSusan RosserEngineering & Physical Sciences Research Council (EPSRC)EP/H019154/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
519114Sandpit: Synthetic integrons for continuous directed evolution of complex genetic ensemblesSusan RosserEngineering & Physical Sciences Research Council (EPSRC)EP/K034359/1RI MOLECULAR CELL & SYSTEMS BIOLOGY