Wei, D., Sun, J., Bolderson, J., Zhong, M., Dalby, M. J. , Cusack, M. , Yin, H. , Fan, H. and Zhang, X. (2017) Continuous fabrication and assembly of spatial cell-laden fibers for a tissue-like construct via a photolithographic-based microfluidic chip. ACS Applied Materials and Interfaces, 9(17), pp. 14606-14617. (doi: 10.1021/acsami.7b00078) (PMID:28157291)
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Abstract
Engineering three-dimensional (3D) scaffolds with in vivo like architecture and function has shown great potential for tissue regeneration. Here we developed a facile microfluidic-based strategy for the continuous fabrication of cell-laden microfibers with hierarchically organized architecture. We show that photolithographically fabricated microfluidic devices offer a simple and reliable way to create anatomically inspired complex structures. Furthermore, the use of photo-cross-linkable methacrylated alginate allows modulation of both the mechanical properties and biological activity of the hydrogels for targeted applications. Via this approach, multilayered hollow microfibers were continuously fabricated, which can be easily assembled in situ, using 3D printing, into a larger, tissue-like construct. Importantly, this biomimetic approach promoted the development of phenotypical functions of the target tissue. As a model to engineer a complex tissue construct, osteon-like fiber was biomimetically engineered, and enhanced vasculogenic and osteogenic expression were observed in the encapsulated human umbilical cord vein endothelial cells and osteoblast-like MG63 cells respectively within the osteon fibers. The capability of this approach to create functional building blocks will be advantageous for bottom-up regeneration of complex, large tissue defects and, more broadly, will benefit a variety of applications in tissue engineering and biomedical research.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Yin, Professor Huabing and Cusack, Professor Maggie and Dalby, Professor Matthew and Bolderson, Mr Jason |
| Authors: | Wei, D., Sun, J., Bolderson, J., Zhong, M., Dalby, M. J., Cusack, M., Yin, H., Fan, H., and Zhang, X. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Engineering College of Science and Engineering > School of Engineering > Biomedical Engineering College of Science and Engineering > School of Geographical and Earth Sciences |
| Journal Name: | ACS Applied Materials and Interfaces |
| Publisher: | American Chemical Society |
| ISSN: | 1944-8244 |
| ISSN (Online): | 1944-8252 |
| Published Online: | 03 February 2017 |
| Copyright Holders: | Copyright © 2017 American Chemical Society |
| First Published: | First published in ACS Applied Materials and Interfaces 9(17): 14606-14617 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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