Neither interleukin-4 receptor α expression on CD4+ T cells, or macrophages and neutrophils is required for protective immunity to Trichinella spiralis

Michels, C. E., Scales, H. E. , Saunders, K. A., McGowan, S., Brombracher, F., Alexander, J. and Lawrence, C. E. (2008) Neither interleukin-4 receptor α expression on CD4+ T cells, or macrophages and neutrophils is required for protective immunity to Trichinella spiralis. Immunology, 128(1pt2), e385-e394. (doi: 10.1111/j.1365-2567.2008.02987.x) (PMID:19191917)

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Abstract

The T helper type 2 (Th2) mediated expulsion of the gastrointestinal nematode Trichinella spiralis requires interleukin-4 receptor α (IL-4Rα) expression on both bone-marrow-derived and non-bone-marrow-derived cells. To more definitively investigate the role of IL-4/IL-13 responsiveness in the development of protective immunity to T. spiralis, cell-specific IL-4Rα signalling on CD4+ T cells (Lckcre IL-4Rα−/flox) and macrophages/neutrophils (LysMcre IL-4Rα−/flox) was analysed on the BALB/c background. Infection of wild-type and control IL-4Rα−/flox mice induced a Th2-type immune response with elevated IL-4 cytokine production, parasite-specific immunoglobulin G1 (IgG1), total IgE, intestinal mastocytosis and enteropathy. In contrast, global IL-4Rα-deficient BALB/c mice showed reduced worm expulsion, antibody production, intestinal mastocytosis and gut pathology. BALB/c mice generated with cell-specific deletion of IL-4Rα on CD4+ T lymphocytes or macrophages/neutrophils, controlled gastrointestinal helminth infection by eliciting a protective immune response comparable to that observed with wild-type and IL-4Rα−/flox controls. Together, this shows that the development of host protective Th2 responses accompanied by parasite loss is independent of IL-4Rα expression on CD4+ T cells and macrophages/neutrophils.

Item Type:Articles
Keywords:Cell-specific interleukin-4 receptor α transgenic mice, gastrointestinal nematodes, mastocytosis, T helper immune response, Trichinella spiralis
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Michels, Dr Chesney and Scales, Dr Hannah
Authors: Michels, C. E., Scales, H. E., Saunders, K. A., McGowan, S., Brombracher, F., Alexander, J., and Lawrence, C. E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Immunology
Publisher:Wiley
ISSN:0019-2805
ISSN (Online):1365-2567
Published Online:16 December 2008
Published Online:01 January 2009

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