Assessment of caveolae/lipid rafts in isolated cells

Callera, G.E., Bruder-Nascimento, T. and Touyz, R.M. (2017) Assessment of caveolae/lipid rafts in isolated cells. In: Touyz, R. M. and Schiffrin, E. L. (eds.) Hypertension: Methods and Protocols. Series: Methods in molecular biology, 1527. Springer: New York, pp. 251-269. ISBN 9781493966233 (doi:10.1007/978-1-4939-6625-7_20)

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This chapter outlines protocols to evaluate protein localization, recruitment or phosphorylation levels in cholesterol/sphingolipids-enriched cell membrane domains and recommends experimental designs with pharmacological tolls to evaluate potential cell functions associated with these domains. We emphasize the need for the combination of several approaches towards understanding the protein components and cellular functions attributed to these distinct microdomains.

Item Type:Book Sections
Glasgow Author(s) Enlighten ID:Touyz, Professor Rhian
Authors: Callera, G.E., Bruder-Nascimento, T., and Touyz, R.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Published Online:24 January 2017

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