Isolation and culture of vascular smooth muscle cells from small and large vessels

Montezano, A. C., Lopes, R. A.M. , Neves, K. B. , Rios, F. and Touyz, R. M. (2017) Isolation and culture of vascular smooth muscle cells from small and large vessels. In: Touyz, R. M. and Schiffrin, E. L. (eds.) Hypertension: Methods and Protocols. Series: Methods in molecular biology (1527). Springer: New York, pp. 349-354. ISBN 9781493966233 (doi:10.1007/978-1-4939-6625-7_27)

Full text not currently available from Enlighten.

Abstract

Primary culture of vascular smooth muscle cells is an important in vitro model for the dissection of molecular mechanisms related to a specific physiological or pathological response at the cellular level. Cultured cells also provide an excellent model to study cell biology. This chapter describes a user-friendly and practical protocol for isolation of vascular smooth muscle cells from small and large vessels by enzymatic dissociation, which can be applied to vessels from different species, including rodents and humans.

Item Type:Book Sections
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Rios, Dr Francisco and Montezano, Dr Augusto and Lopes, Dr Rheure and Touyz, Professor Rhian and Neves, Dr Karla Bianca
Authors: Montezano, A. C., Lopes, R. A.M., Neves, K. B., Rios, F., and Touyz, R. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Publisher:Springer
ISBN:9781493966233
Published Online:24 January 2017

University Staff: Request a correction | Enlighten Editors: Update this record