Montezano, A. C., Lopes, R. A.M. , Neves, K. B. , Rios, F. and Touyz, R. M. (2017) Isolation and culture of vascular smooth muscle cells from small and large vessels. In: Touyz, R. M. and Schiffrin, E. L. (eds.) Hypertension: Methods and Protocols. Series: Methods in molecular biology (1527). Springer: New York, pp. 349-354. ISBN 9781493966233 (doi: 10.1007/978-1-4939-6625-7_27)
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Abstract
Primary culture of vascular smooth muscle cells is an important in vitro model for the dissection of molecular mechanisms related to a specific physiological or pathological response at the cellular level. Cultured cells also provide an excellent model to study cell biology. This chapter describes a user-friendly and practical protocol for isolation of vascular smooth muscle cells from small and large vessels by enzymatic dissociation, which can be applied to vessels from different species, including rodents and humans.
| Item Type: | Book Sections |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Rios, Dr Francisco and Montezano, Dr Augusto and Alves Moreira Lopes, Dr Rheure and Touyz, Professor Rhian and Neves, Dr Karla |
| Authors: | Montezano, A. C., Lopes, R. A.M., Neves, K. B., Rios, F., and Touyz, R. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
| Publisher: | Springer |
| ISBN: | 9781493966233 |
| Published Online: | 24 January 2017 |
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