Tissue proteomics in vascular disease

Albalat, A., Mullen, W. , Husi, H. and Mischak, H. (2017) Tissue proteomics in vascular disease. In: Touyz, R. M. and Schiffrin, E. L. (eds.) Hypertension: Methods and Protocols. Series: Methods in molecular biology (1527). Springer New York: New York, pp. 53-60. ISBN 9781493966233 (doi: 10.1007/978-1-4939-6625-7_4)

Abstract

In vascular diseases, as in many other pathophysiological conditions, tissue proteins are subjected to a number of different changes such as protein expression, posttranslational modifications, and proteolytic cleavage. For this reason, the study of the tissue proteome is becoming an increasingly important tool in biological and clinical research. In this chapter, we describe in detail the methodology for the analysis of tryptic digested peptides from aortic tissue extracts from mice with the aim to elucidate differences in the proteome between control and case or vascular diseased tissue samples. The method encompasses the analysis of these complex extracts in a single chromatographic run allowing for increased reproducibility. We also outline the main workflow for the processing of the generated data by statistical and bioinformatic analysis.

• Item Type: Book Sections
• Additional Information: This work was supported in part by European grant HEALTH- 2011-278249-EU-MASCARA.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Albalat, Dr Amaya and Mullen, Dr Bill and Husi, Dr Holger and Mischak, Professor Harald
• Authors: Albalat, A., Mullen, W., Husi, H., and Mischak, H.
• Subjects: ?? FASP method ??; ?? MS/MS data analysis ??; ?? Tissue proteomics ??
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Methods in Molecular Biology
• Publisher: Springer New York
• ISSN: 1064-3745
• ISSN (Online): 1940-6029
• ISBN: 9781493966233