Tsiropoulou, S., McBride, M. and Padmanabhan, S. (2017) Urine metabolomics in hypertension research. In: Touyz, R. M. and Schiffrin, E. L. (eds.) Hypertension: Methods and Protocols. Series: Methods in molecular biology, 1527. Springer: New York, pp. 61-68. ISBN 9781493966233 (doi: 10.1007/978-1-4939-6625-7_5)
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Publisher's URL: https://www.springer.com/gb/book/9781493966233
Abstract
Functional genomics requires an understanding of the complete network of changes within an organism by extensive measurements of moieties from mRNA, proteins, and metabolites. Metabolomics utilizes analytic chemistry tools to profile the complete spectrum of metabolites found in a tissue, cells, or biofluids using a wide range of tools from infrared spectroscopy, fluorescence spectroscopy, NMR spectroscopy, and mass spectrometry. In this protocol, we outline a procedure for performing metabolomic analysis of urine samples using liquid chromatography–mass spectrometry (LC-MS). We outline the advantages of using this approach and summarize some of the early promising studies in cardiovascular diseases using this approach.
Item Type: | Book Sections |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Tsiropoulou, Miss Sofia and McBride, Dr Martin and Padmanabhan, Professor Sandosh |
Authors: | Tsiropoulou, S., McBride, M., and Padmanabhan, S. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
Publisher: | Springer |
ISBN: | 9781493966233 |
Published Online: | 24 January 2017 |
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