Isolation and differentiation of human macrophages

Rios, F. J., Touyz, R. M. and Montezano, A. C. (2017) Isolation and differentiation of human macrophages. In: Touyz, R. M. and Schiffrin, E. L. (eds.) Hypertension: Methods and Protocols. Series: Methods in molecular biology, 1527. Springer: New York, pp. 311-320. ISBN 9781493966233 (doi:10.1007/978-1-4939-6625-7_24)

Rios, F. J., Touyz, R. M. and Montezano, A. C. (2017) Isolation and differentiation of human macrophages. In: Touyz, R. M. and Schiffrin, E. L. (eds.) Hypertension: Methods and Protocols. Series: Methods in molecular biology, 1527. Springer: New York, pp. 311-320. ISBN 9781493966233 (doi:10.1007/978-1-4939-6625-7_24)

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Publisher's URL: https://www.springer.com/gb/book/9781493966233

Abstract

Macrophage subtypes display protective or pathogenic activities in vascular lesions. They recognize and engulf modified lipids, accumulate in the arterial intima, contributing to the atherosclerosis development. In the heart, vascular tissues and perivascular adipose tissues, there is increasing evidence that macrophages play a role in endothelial dysfunction, vascular inflammation and remodeling. In this chapter we describe protocols to isolate human monocytes from peripheral blood mononuclear cell and how to differentiate them into macrophages by using growth factors (granulocyte-macrophage colony-stimulating factor—GM-CSF or colony-stimulating factor—M-CSF) or by different cell culture medium concentration. We also describe how to indentify different macrophage activation phenotypes.

Item Type:Book Sections
Status:Published
Glasgow Author(s) Enlighten ID:Montezano, Dr Augusto and Touyz, Professor Rhian and Rios, Dr Francisco
Authors: Rios, F. J., Touyz, R. M., and Montezano, A. C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Publisher:Springer
ISBN:9781493966233
Published Online:24 January 2017

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