A plasma membrane template for macropinocytic cups

Veltman, D. M., Williams, T. D., Bloomfield, G., Chen, B.-C., Betzig, E., Insall, R. and Kay, R. R. (2016) A plasma membrane template for macropinocytic cups. eLife, 5, e20085. (doi:10.7554/eLife.20085) (PMID:27960076) (PMCID:PMC5154761)

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Abstract

Macropinocytosis is a fundamental mechanism that allows cells to take up extracellular liquid into large vesicles. It critically depends on the formation of a ring of protrusive actin beneath the plasma membrane, which develops into the macropinocytic cup. We show that macropinocytic cups in Dictyostelium are organised around coincident intense patches of PIP3, active Ras and active Rac. These signalling patches are invariably associated with a ring of active SCAR/WAVE at their periphery, as are all examined structures based on PIP3 patches, including phagocytic cups and basal waves. Patch formation does not depend on the enclosing F-actin ring, and patches become enlarged when the RasGAP NF1 is mutated, showing that Ras plays an instructive role. New macropinocytic cups predominantly form by splitting from existing ones. We propose that cup-shaped plasma membrane structures form from self-organizing patches of active Ras/PIP3, which recruit a ring of actin nucleators to their periphery.

Item Type:Articles
Additional Information:Funder: Biotechnology and Biological Sciences Research Council BB/K009699/1 Douwe M Veltman Robert R Kay. Medical Research Council U105115237 Robert R Kay.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Insall, Professor Robert
Authors: Veltman, D. M., Williams, T. D., Bloomfield, G., Chen, B.-C., Betzig, E., Insall, R., and Kay, R. R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:eLife
Publisher:eLife Sciences Publications
ISSN:2050-084X
ISSN (Online):2050-084X
Copyright Holders:Copyright © 2016 Veltman et al.
First Published:First published in eLife 5:e20085
Publisher Policy:Reproduced under a Creative Commons License

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