Profiling the essential nature of lipid metabolism in asexual blood and gametocyte stages of Plasmodium falciparum

Gulati, S. et al. (2015) Profiling the essential nature of lipid metabolism in asexual blood and gametocyte stages of Plasmodium falciparum. Cell Host and Microbe, 18(3), pp. 371-381. (doi:10.1016/j.chom.2015.08.003) (PMID:26355219) (PMCID:PMC4567697)

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Abstract

During its life cycle, Plasmodium falciparum undergoes rapid proliferation fueled by de novo synthesis and acquisition of host cell lipids. Consistent with this essential role, Plasmodium lipid synthesis enzymes are emerging as potential drug targets. To explore their broader potential for therapeutic interventions, we assayed the global lipid landscape during P. falciparum sexual and asexual blood stage (ABS) development. Using liquid chromatography-mass spectrometry, we analyzed 304 lipids constituting 24 classes in ABS parasites, infected red blood cell (RBC)-derived microvesicles, gametocytes, and uninfected RBCs. Ten lipid classes were previously uncharacterized in P. falciparum, and 70%–75% of the lipid classes exhibited changes in abundance during ABS and gametocyte development. Utilizing compounds that target lipid metabolism, we affirmed the essentiality of major classes, including triacylglycerols. These studies highlight the interplay between host and parasite lipid metabolism and provide a comprehensive analysis of P. falciparum lipids with candidate pathways for drug discovery efforts.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Marti, Professor Matthias
Authors: Gulati, S., Ekland, E. H., Ruggles, K. V., Chan, R. B., Jayabalasingham, B., Zhou, B., Mantel, P.-Y., Lee, M. C.S., Spottiswoode, N., Coburn-Flynn, O., Hjelmqvist, D., Worgall, T. S., Marti, M., Di Paolo, G., and Fidock, D. A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Cell Host and Microbe
Publisher:Elsevier
ISSN:1931-3128
ISSN (Online):1934-6069
Published Online:09 September 2015

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