Isolation and identification of intestinal myeloid cells

Scott, C. L., Bain, C. C. and Mowat, A. M. (2017) Isolation and identification of intestinal myeloid cells. In: Clausen, B. E. and Laman, J. D. (eds.) Inflammation: Methods and Protocols. Series: Methods in molecular biology (1559). Humana Press, pp. 223-239. ISBN 9781493967841 (doi:10.1007/978-1-4939-6786-5_15)

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Publisher's URL: https://link.springer.com/book/10.1007%2F978-1-4939-6786-5

Abstract

The identification of conventional dendritic cells (cDCs) and macrophages (mϕ) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from each other and from other intestinal myeloid cells. Here we detail the protocols we have developed to isolate live leukocytes from steady state mouse small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs, monocytes and macrophages in such preparations.

Item Type:Book Sections
Status:Published
Glasgow Author(s) Enlighten ID:Mowat, Professor Allan and Bain, Mr Calum
Authors: Scott, C. L., Bain, C. C., and Mowat, A. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Publisher:Humana Press
ISBN:9781493967841
Published Online:07 January 2017

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