The architecture of an Okazaki fragment-processing holoenzyme from the archaeon Sulfolobus solfataricus

Cannone, G., Xu, Y., Beattie, T. R., Bell, S. D. and Spagnolo, L. (2015) The architecture of an Okazaki fragment-processing holoenzyme from the archaeon Sulfolobus solfataricus. Biochemical Journal, 465(2), pp. 239-245. (doi:10.1042/BJ20141120) (PMID:25299633)

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Abstract

DNA replication on the lagging strand occurs via the synthesis and maturation of Okazaki fragments. In archaea and eukaryotes, the enzymatic activities required for this process are supplied by a replicative DNA polymerase, Flap endonuclease 1 (Fen1) and DNA ligase 1 (Lig1). These factors interact with the sliding clamp PCNA (proliferating cell nuclear antigen) providing a potential means of co-ordinating their sequential actions within a higher order assembly. In hyperthermophilic archaea of the Sulfolobus genus, PCNA is a defined heterotrimeric assembly and each subunit interacts preferentially with specific client proteins. We have exploited this inherent asymmetry to assemble a PCNA-polymerase-Fen1-ligase complex on DNA and have visualized it by electron microscopy. Our studies reveal the structural basis of co-occupancy of a single PCNA ring by the three distinct client proteins.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Spagnolo, Dr Laura
Authors: Cannone, G., Xu, Y., Beattie, T. R., Bell, S. D., and Spagnolo, L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Biochemical Journal
Publisher:Portland Press
ISSN:0264-6021
ISSN (Online):1470-8728
Published Online:09 October 2014
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Biochemical Journal 465(2):239-245
Publisher Policy:Reproduced under a Creative Commons License

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