Fu, G., Perona-Wright, G. and Barker, D. C. (1998) Leishmania braziliensis: characterisation of a complex specific subtelomeric repeat sequence and its use in the detection of parasites. Experimental Parasitology, 90(3), pp. 236-243. (doi: 10.1006/expr.1998.4326) (PMID:9806868)
Full text not currently available from Enlighten.
Abstract
A 1.6-kb tandem repeat sequence had previously been identified in the subtelomeric region of mini- and megabase chromosomes from Leishmania braziliensis. Southern hybridisation was used to demonstrate that the repeat is complex specific. The sequence was characterised in strains representing four species of the L. braziliensis complex. This data allowed an assessment of the evolutionary relationship of the four species. PCR primers targeted to the repeat amplify only DNA from species of the L. braziliensis complex. Titration assays indicate that a minimum of 50 fg of parasite DNA can be detected by PCR alone. Southern hybridisation increases the limit of detection to 5 fg. Interspecies variation in the repeat sequence enabled restriction enzyme digestion of PCR products to distinguish individual species within the L. braziliensis complex.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Perona-Wright, Dr Georgia |
| Authors: | Fu, G., Perona-Wright, G., and Barker, D. C. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Experimental Parasitology |
| Publisher: | Elsevier |
| ISSN: | 0014-4894 |
| ISSN (Online): | 1090-2449 |
| Published Online: | 15 April 2002 |
University Staff: Request a correction | Enlighten Editors: Update this record
Altmetric
Altmetric