The Porphyromonas gingivalis hemagglutinins HagB and HagC are major mediators of adhesion and biofilm formation

Connolly, E., Millhouse, E., Doyle, R., Culshaw, S. , Ramage, G. and Moran, G.P. (2017) The Porphyromonas gingivalis hemagglutinins HagB and HagC are major mediators of adhesion and biofilm formation. Molecular Oral Microbiology, 32(1), pp. 35-47. (doi:10.1111/omi.12151) (PMID:28051836)

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Abstract

Porphyromonas gingivalis is a bacterium associated with chronic periodontitis that possesses a family of genes encoding hemagglutinins required for heme acquisition. In this study we generated ΔhagB and ΔhagC mutants in strain W83 and demonstrate that both hagB and hagC are required for adherence to oral epithelial cells. Unexpectedly, a double ΔhagB/ΔhagC mutant had less severe adherence defects than either of the single mutants, but was found to exhibit increased expression of the gingipain-encoding genes rgpA and kgp, suggesting that a ΔhagB/ΔhagC mutant is only viable in populations of cells that exhibit increased expression of genes involved in heme acquisition. Disruption of hagB in the fimbriated strain ATCC33277 demonstrated that HagB is also required for stable attachment of fimbriated bacteria to oral epithelial cells. Mutants of hagC were also found to form defective single and multi-species biofilms that had reduced biomass relative to biofilms formed by the wild-type strain. This study highlights the hitherto unappreciated importance of these genes in oral colonization and biofilm formation.

Item Type:Articles
Additional Information:EC was supported by a PhD studentship, part funded by Trinity College Dublin and The Dublin Dental University Hospital. RD was supported by a Glaxo-Smith-Kline (GSK) Ireland Summer studentship. EM was supported by a studentship from the BBSRC, UK.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Culshaw, Dr Shauna and Ramage, Professor Gordon and Millhouse, Dr Emma
Authors: Connolly, E., Millhouse, E., Doyle, R., Culshaw, S., Ramage, G., and Moran, G.P.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Molecular Oral Microbiology
Publisher:Wiley
ISSN:2041-1006
ISSN (Online):2041-1014
Published Online:04 February 2016
Copyright Holders:Copyright © 2015 John Wiley & Sons A/S
First Published:First published in Molecular Oral Microbiology 32(1):35-47
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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