Exploring host-pathogen interactions through genome wide protein microarray analysis

Scietti, L. et al. (2016) Exploring host-pathogen interactions through genome wide protein microarray analysis. Scientific Reports, 6, 27996. (doi: 10.1038/srep27996) (PMID:27302108) (PMCID:PMC4908583)

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Abstract

During bacterial pathogenesis extensive contacts between the human and the bacterial extracellular proteomes take place. The identification of novel host-pathogen interactions by standard methods using a case-by-case approach is laborious and time consuming. To overcome this limitation, we took advantage of large libraries of human and bacterial recombinant proteins. We applied a large-scale protein microarray-based screening on two important human pathogens using two different approaches: (I) 75 human extracellular proteins were tested on 159 spotted Staphylococcus aureus recombinant proteins and (II) Neisseria meningitidis adhesin (NadA), an important vaccine component against serogroup B meningococcus, was screened against ≈2300 spotted human recombinant proteins. The approach presented here allowed the identification of the interaction between the S. aureus immune evasion protein FLIPr (formyl-peptide receptor like-1 inhibitory protein) and the human complement component C1q, key players of the offense-defense fighting; and of the interaction between meningococcal NadA and human LOX-1 (low-density oxidized lipoprotein receptor), an endothelial receptor. The novel interactions between bacterial and human extracellular proteins here presented might provide a better understanding of the molecular events underlying S. aureus and N. meningitidis pathogenesis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Haag, Dr Andreas
Authors: Scietti, L., Sampieri, K., Pinzuti, I., Bartolini, E., Benucci, B., Liguori, A., Haag, A. F., Lo Surdo, P., Pansegrau, W., Nardi-Dei, V., Santini, L., Arora, S., Leber, X., Rindi, S., Savino, S., Costantino, P., Maione, D., Merola, M., Speziale, P., Bottomley, M. J., Bagnoli, F., Masignani, V., Pizza, M., Scharenberg, M., Schlaeppi, J.-M., Nissum, M., and Liberatori, S.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Scientific Reports
Publisher:Nature Research
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Scientific Reports 6:27996
Publisher Policy:Reproduced under a creative commons license

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