Characterisation of species and diversity of Anopheles gambiae Keele Colony

Ranford-Cartwright, L. C., McGeechan, S., Inch, D., Smart, G., Richterová, L. and Mwangi, J. M. (2016) Characterisation of species and diversity of Anopheles gambiae Keele Colony. PLoS ONE, 11(12), e0168999. (doi:10.1371/journal.pone.0168999) (PMID:28033418) (PMCID:PMC5199079)

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Abstract

Anopheles gambiae sensu stricto was recently reclassified as two species, An. coluzzii and An. gambiae s.s., in wild-caught mosquitoes, on the basis of the molecular form, denoted M or S, of a marker on the X chromosome. The An. gambiae Keele line is an outbred laboratory colony strain that was developed around 12 years ago by crosses between mosquitoes from 4 existing An. gambiae colonies. Laboratory colonies of mosquitoes often have limited genetic diversity because of small starting populations (founder effect) and subsequent fluctuations in colony size. Here we describe the characterisation of the chromosomal form(s) present in the Keele line, and investigate the diversity present in the colony using microsatellite markers on chromosome 3. We also characterise the large 2La inversion on chromosome 2. The results indicate that only the M-form of the chromosome X marker is present in the Keele colony, which was unexpected given that 3 of the 4 parent colonies were probably S-form. Levels of diversity were relatively high, as indicated by a mean number of microsatellite alleles of 6.25 across 4 microsatellites, in at least 25 mosquitoes. Both karyotypes of the inversion on chromosome 2 (2La/2L+a) were found to be present at approximately equal proportions. The Keele colony has a mixed M- and S-form origin, and in common with the PEST strain, we propose continuing to denote it as an An. gambiae s.s. line.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ranford-Cartwright, Dr Lisa and Mwangi, Dr Jonathan
Authors: Ranford-Cartwright, L. C., McGeechan, S., Inch, D., Smart, G., Richterová, L., and Mwangi, J. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2016 Ranford-Cartwright et al.
First Published:First published in PLoS ONE 11(12):e0168999
Publisher Policy:Reproduced under a creative commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
526791Molecular Functions in DiseaseDarren MoncktonWellcome Trust (WELLCOME)089886/Z/09/ZRI MOLECULAR CELL & SYSTEMS BIOLOGY
534421Genetic analysis of growth rates in Plasmodium falciparumLisa Ranford-CartwrightWellcome Trust (WELLCOME)091791/Z/10/ZIII - PARASITOLOGY