Recent advances in expanding the coverage of the lipidome

Tumanov, S. and Kamphorst, J. J. (2017) Recent advances in expanding the coverage of the lipidome. Current Opinion in Biotechnology, 43, pp. 127-133. (doi:10.1016/j.copbio.2016.11.008) (PMID:27915214) (PMCID:PMC5312421)

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Abstract

The lipidome comprises a large array of molecules with diverse physicochemical properties. Lipids are structural components of cells, act as a source of energy, and function as signaling mediators. Alterations in lipid metabolism are involved in the onset and progression of a variety of diseases, including metabolic syndrome and cancer. Because of this, interest in lipidomics, the comprehensive characterization of the lipidome by mass spectrometry, has intensified in recent years. However, obtaining a truly complete overview of all lipids in a sample has remained very challenging due to their enormous structural diversity. Here, we provide an overview of the collection of analytical approaches used to study various lipid classes, emphasizing innovations in sample preparation and liquid chromatography–mass spectrometry (LC–MS). Additionally, we provide practical suggestions for increasing the coverage of the lipidome.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tumanov, Mr Sergey and Kamphorst, Dr Jurre
Authors: Tumanov, S., and Kamphorst, J. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Current Opinion in Biotechnology
Publisher:Elsevier
ISSN:0958-1669
ISSN (Online):1879-0429
Published Online:01 December 2016

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
655161The effect of tumour microenvironment on the metabolism of pancreatic cancer cellsJurre KamphorstCancer Research UK (CAN-RES-UK)C50242/A17728ICS - BEATSON INSTITUTE FOR CANCER RES.