ROCK signalling induced gene expression changes in mouse pancreatic ductal adenocarcinoma cells

Rath, N., Kalna, G., Clark, W. and Olson, M. F. (2016) ROCK signalling induced gene expression changes in mouse pancreatic ductal adenocarcinoma cells. Scientific Data, 3, p. 160101. (doi: 10.1038/sdata.2016.101) (PMID:27824338) (PMCID:PMC5100681)

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Abstract

The RhoA and RhoC GTPases act via the ROCK1 and ROCK2 kinases to promote actomyosin contraction, resulting in directly induced changes in cytoskeleton structures and altered gene transcription via several possible indirect routes. Elevated activation of the Rho/ROCK pathway has been reported in several diseases and pathological conditions, including disorders of the central nervous system, cardiovascular dysfunctions and cancer. To determine how increased ROCK signalling affected gene expression in pancreatic ductal adenocarcinoma (PDAC) cells, we transduced mouse PDAC cell lines with retroviral constructs encoding fusion proteins that enable conditional activation of ROCK1 or ROCK2, and subsequently performed RNA sequencing (RNA-Seq) using the Illumina NextSeq 500 platform. We describe how gene expression datasets were generated and validated by comparing data obtained by RNA-Seq with RT-qPCR results. Activation of ROCK1 or ROCK2 signalling induced significant changes in gene expression that could be used to determine how actomyosin contractility influences gene transcription in pancreatic cancer.

Item Type:Articles
Additional Information:Funding for this project was from Cancer Research U.K. (A18276).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Clark, Mr William and Rath, Dr Nicola and Kalna, Dr Gabriela and Olson, Professor Michael
Authors: Rath, N., Kalna, G., Clark, W., and Olson, M. F.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Scientific Data
Publisher:Nature Publishing Group
ISSN:2052-4463
ISSN (Online):2052-4463
Published Online:08 November 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Scientific Data 3:160101
Publisher Policy:Reproduced under a Creative Commons License

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