Photopatterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions

Yin, H. B. , Brown, T., Greef, R., Mailis, S., Eason, R., Wilkinson, J. S. and Melvin, T. (2004) Photopatterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions. In: Biophotonics New Frontier: From Genome to Proteome, Strasbourg, France, 26 Apr 2004, ISBN 9780819453846 (doi:10.1117/12.545736)

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Abstract

A method is presented for the covalent attachment of oligonucleotides to silicon (100) surfaces patterned with micronscale features. UV light exposure of hydrogen-terminated silicon (100) coated with alkenes functionalized with Nhydroxysuccinimide ester groups results in Si-C bonded monolayers. The N-hydroxysuccinimide ester surfaces act as a template for the subsequent covalent attachment of DNA oligonucleotides. In order to create patterns of surface attached DNA oligonucleotides with high density, the surface attachment chemistry has been investigated and optimised. Micron-scale patterning of surfaces was achieved by exposure with UV laser light via a mask. DNA oligonucleotide patterns, with feature sizes of several microns, were reliably produced over large areas. The patterned surfaces were characterised with scanning electron microscopy, epifluorescence microscopy and ellipsometry. Hybridisation with fluorescent label- and gold nanoparticle-conjugates of the complementary oligonucleotide is achieved. The methods offer reliable approaches for the creation of micron-scale motifs of DNA on surfaces.

Item Type:Conference Proceedings
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Yin, Professor Huabing
Authors: Yin, H. B., Brown, T., Greef, R., Mailis, S., Eason, R., Wilkinson, J. S., and Melvin, T.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
ISSN:0277-786X
ISBN:9780819453846

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