Osteogenic and Bactericidal Surfaces from Hydrothermal Titania Nanowires on Titanium Substrates

Tsimbouri, P.M. , Holloway, N., Fisher, L., Sjostrom, T., Nobbs, A.H., Meek, R.M.D., Su, B. and Dalby, M.J. (2016) Osteogenic and Bactericidal Surfaces from Hydrothermal Titania Nanowires on Titanium Substrates. The British Orthopaedic Research Society (BORS) Annual Conference, Glasgow, UK, 5-6 Sept 2016.

Abstract

Nanotopographical cues on Ti surfaces have been shown to elicit different cell responses such as differentiation and selective growth. Bone remodelling is a continuous process requiring specific cues for optimal bone growth and implant fixation. In addition, the prevention of biofilm formation on surgical implants is a major challenge. We have identified nanopatterns on Ti surfaces that would be optimal for both bone remodelling and for reducing risk of bacterial infection. We used primary human osteoblast/osteoclast co-cultures and seeded them on flat Ti and three Ti nanosurfaces with increasing degrees of roughness, manufactured using anodisation under alkaline conditions (for 2, 2.5 and 3 hours). Cell growth and behaviour was assessed by scanning electron microscopy (SEM), immunofluorescence microscopy, histochemistry and quantitative RT-PCR methods. Bacterial growth on the nanowire surfaces was also assessed by confocal microscopy and SEM. From the three surfaces tested, the 2 h nanowire surface supported osteoblast and, to a lesser extent, osteoclast growth and differentiation. Bacterial viability was significantly reduced on the 2h surface. Hence the 2 h surface provided optimal bone remodelling conditions while reducing infection risk, making it a favourable candidate for future implant surfaces.

• Item Type: Conference or Workshop Item
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Tsimbouri, Dr Monica and Dalby, Professor Matthew
• Authors: Tsimbouri, P.M., Holloway, N., Fisher, L., Sjostrom, T., Nobbs, A.H., Meek, R.M.D., Su, B., and Dalby, M.J.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
61557	1	Multiscale topographical modulation of cells and bacteria for next generation orthopaedic implants	Matthew Dalby	Engineering & Physical Sciences Research Council (EPSRC)	EP/K034898/1	RI MOLECULAR CELL & SYSTEMS BIOLOGY