Osteogenic and Bactericidal Surfaces from Hydrothermal Titania Nanowires on Titanium Substrates

Tsimbouri, P.M. , Holloway, N., Fisher, L., Sjostrom, T., Nobbs, A.H., Meek, R.M.D., Su, B. and Dalby, M.J. (2016) Osteogenic and Bactericidal Surfaces from Hydrothermal Titania Nanowires on Titanium Substrates. The British Orthopaedic Research Society (BORS) Annual Conference, Glasgow, UK, 5-6 Sept 2016.

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Publisher's URL: http://www.bjjprocs.boneandjoint.org.uk/content/98-B/SUPP_16/24

Abstract

Nanotopographical cues on Ti surfaces have been shown to elicit different cell responses such as differentiation and selective growth. Bone remodelling is a continuous process requiring specific cues for optimal bone growth and implant fixation. In addition, the prevention of biofilm formation on surgical implants is a major challenge. We have identified nanopatterns on Ti surfaces that would be optimal for both bone remodelling and for reducing risk of bacterial infection. We used primary human osteoblast/osteoclast co-cultures and seeded them on flat Ti and three Ti nanosurfaces with increasing degrees of roughness, manufactured using anodisation under alkaline conditions (for 2, 2.5 and 3 hours). Cell growth and behaviour was assessed by scanning electron microscopy (SEM), immunofluorescence microscopy, histochemistry and quantitative RT-PCR methods. Bacterial growth on the nanowire surfaces was also assessed by confocal microscopy and SEM. From the three surfaces tested, the 2 h nanowire surface supported osteoblast and, to a lesser extent, osteoclast growth and differentiation. Bacterial viability was significantly reduced on the 2h surface. Hence the 2 h surface provided optimal bone remodelling conditions while reducing infection risk, making it a favourable candidate for future implant surfaces.

Item Type:Conference or Workshop Item
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tsimbouri, Dr Penelope and Dalby, Professor Matthew
Authors: Tsimbouri, P.M., Holloway, N., Fisher, L., Sjostrom, T., Nobbs, A.H., Meek, R.M.D., Su, B., and Dalby, M.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
615571Multiscale topographical modulation of cells and bacteria for next generation orthopaedic implantsMatthew DalbyEngineering & Physical Sciences Research Council (EPSRC)EP/K034898/1RI MOLECULAR CELL & SYSTEMS BIOLOGY