The antiviral RNAi response in vector and non-vector cells against orthobunya viruses

Dietrich, I., Shi, X. , McFarlane, M. , Watson, M., Blomström, A.-L., Skelton, J. K., Kohl, A. , Elliott, R. M. and Schnettler, E. (2017) The antiviral RNAi response in vector and non-vector cells against orthobunya viruses. PLoS Neglected Tropical Diseases, 11(1), e0005272. (doi:10.1371/journal.pntd.0005272) (PMID:28060823) (PMCID:PMC5245901)

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Abstract

Background: Vector arthropods control arbovirus replication and spread through antiviral innate immune responses including RNA interference (RNAi) pathways. Arbovirus infections have been shown to induce the exogenous small interfering RNA (siRNA) and Piwi-interacting RNA (piRNA) pathways, but direct antiviral activity by these host responses in mosquito cells has only been demonstrated against a limited number of positive-strand RNA arboviruses. For bunyaviruses in general, the relative contribution of small RNA pathways in antiviral defences is unknown. Methodology/Principal Findings: The genus Orthobunyavirus in the Bunyaviridae family harbours a diverse range of mosquito-, midge- and tick-borne arboviruses. We hypothesized that differences in the antiviral RNAi response in vector versus non-vector cells may exist and that could influence viral host range. Using Aedes aegypti-derived mosquito cells, mosquito-borne orthobunyaviruses and midge-borne orthobunyaviruses we showed that bunyavirus infection commonly induced the production of small RNAs and the effects of the small RNA pathways on individual viruses differ in specific vector-arbovirus interactions. Conclusions/Significance: These findings have important implications for our understanding of antiviral RNAi pathways and orthobunyavirus-vector interactions and tropism.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Mcdonald, Dr Melanie and Elliott, Professor Richard and Skelton, Miss Jessica and Blomstrom, Dr Anne-Lie and Shi, Dr Xiaohong and Dietrich, Dr Isabelle and Schnettler, Dr Esther and Kohl, Professor Alain
Authors: Dietrich, I., Shi, X., McFarlane, M., Watson, M., Blomström, A.-L., Skelton, J. K., Kohl, A., Elliott, R. M., and Schnettler, E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:PLoS Neglected Tropical Diseases
Publisher:Public Library of Science
ISSN:1935-2727
ISSN (Online):1935-2735
Copyright Holders:Copyright © 2017 Dietrich et al.
First Published:First published in PLoS Neglected Tropical Diseases 11(1):e0005272
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656551Arbovirus interactions with arthropod hostsAlain KohlMedical Research Council (MRC)MC_UU_12014/8MVLS III - CENTRE FOR VIRUS RESEARCH
635361Molecular analyses of arbovirus-host interactionsMassimo PalmariniWellcome Trust (WELLCOME)099220/B/12/ZMVLS III - CENTRE FOR VIRUS RESEARCH

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