Direct interaction of aminopeptidase A with recombination site DNA in Xer site-specific recombination

Alen, C., Sherratt, D. J. and Colloms, S. D. (1997) Direct interaction of aminopeptidase A with recombination site DNA in Xer site-specific recombination. EMBO Journal, 16(17), pp. 5188-5197. (doi:10.1093/emboj/16.17.5188) (PMID:9311979) (PMCID:PMC1170151)

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Abstract

Xer site‐specific recombination at ColE1 cer converts plasmid multimers into monomers, thus ensuring the heritable stability of ColE1. Two related recombinase proteins, XerC and XerD, catalyse the strand exchange reaction at a 30 bp recombination core site. In addition, two accessory proteins, PepA and ArgR, are required for recombination at cer. These two accessory proteins are thought to act at 180 bp of accessory sequences adjacent to the cer recombination core to ensure that recombination only occurs between directly repeated sites on the same molecule. Here, we demonstrate that PepA and ArgR interact directly with cer, forming a complex in which the accessory sequences of two cer sites are interwrapped approximately three times in a right‐handed fashion. We present a model for this synaptic complex, and propose that strand exchange can only occur after the formation of this complex.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Colloms, Dr Sean
Authors: Alen, C., Sherratt, D. J., and Colloms, S. D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:EMBO Journal
Publisher:EMBO Press
ISSN:0261-4189
ISSN (Online):1460-2075

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